Male and female beagle dogs (7.5 - 13.5 kg) were placed individually in a cage (70 x 70 x 70 cm). NE-100 (0.03, 0.1 and 0.3 mg/kg) was given orally 10 min before the administration of PCP (0.6 mg/kg, s.c.), then the animals were observed for abnormal behavior every 10 min for 60 min. Abnormal behavior was scored according to the following criteria.

Cells were seeded into 96-well plates for optical performance in the fluorescent cell-based assay in 100 μl complete medium in the presence or absence of different concentrations of NE-100. The plate was incubated for 24 h in a humidified atmosphere 5% CO2 at 37°C and then 100 μl substrate mix in assay buffer added. A 10 μl aliquot of lysis solution was added to untreated wells to estimate total LDH. Plates were kept protected from light for 10 min at room temperature, and then 50 μl stop solution was added to all wells. The fluorescence was recorded using a LS55 Luminescence Spectrometer Perkin Elmer (excitation at 560 nm; emission at 590 nm).