A single oral dose of 1200 mg pasiniazide and dipasic tablets was given according to a cross over design.

Minimum inhibitory concentration (MIC) determination. Inocula were prepared from actively growing bacteria collected from LJ slants or Middlebrook 7H10 agar plates. Inocula were adjusted with saline to a cell density of 1.5 x 10^8 cells/mL (0.5 McFarland standard) and were then diluted (1:200) using CA-MHB for RGM or CAMHB+ 5% ADC supplement (CA-MHB-S) for SGM. Dipasic (Pasiniazid) were serially diluted two-fold in 100 μL of CA-MHB or CA-MHB-S. The final reaction volume was 200 μL (100 μL of the antibiotic solution and 100 μL of the bacterial suspension). Three negative controls were used as part of this study: medium without antibiotics was used to determine the addition time for alamarBlue; Medium without inoculum was used to determine the interference to alamarBlue by the medium; and a series of concentration gradients for Dipasic were used to determine the interference to alamarBlue by the drug–medium mixture colour. The plates were sealed in individual Ziploc bags and were incubated at 37 °C. After 24 h for RGM or 6 days for SGM, the first drug-free growth control wells were examined using an indicator (20 μL of alamarBlue and 50 μL of sterile 5% Tween-80). The plateswere then re-incubated for 24 h. If the control well changed to a pink colour, all of the wells containing drugs were supplemented with the indicator. After a further 24 h of incubation, the colour of all of the wells was recorded. Each MIC for the tested drug was recorded on a daily basis between the third and sixth days for RGM and between the seventh and eleventh days for SGM. The MIC was defined as the lowest drug concentration that prevented a change in colour.