2,000-3,000 mg daily in two divided doses as Phytic Acid calcium/magnesium salt.

Human cell lines HT-29, SW-480 and SW-620 derived from colorectal carcinoma was used for activity evaluation. Stock solutions of IP6 (Phytic Acid) and Ins were prepared in PBS without ions and stored in a refrigerator until use. Before each experiment, stocks were diluted to the final concentration in Nutrient Mixture F-12 Ham (N-4888) with 1% FBS and 1% L-glutamine. For experiments, the cells were seeded into cultivation flasks (Nunclon, Roskilde, Denmark) at the density of 1x10^5 cells/ml (in 20 ml total volume) or 96-well plates (Nunclon, Roskilde, Denmark; Greiner bio-one, Germany) at concentration of 6,000 cells/well, always with the first column of wells without cells (blank) and allowed to grow overnight. After 24 h, the medium was aspirated and cells were exposed to the freshly prepared medium containing IP6 (0.2, 1 and 5 mM), Ins or their combination for 24, 48 or 72 h. At the end of each incubation, the medium was removed; cells were washed twice and exposed to medium with 100 μl of WST-1. After 2 h, the changes in absorbance were recorded by a photometer SPEKTRAFluor Plus, Tecan (Salzburg, Austria) at 450 nm with 650 nm of reference wavelength.