Iodophthalein was used as an iodine-containing X-ray contrast agent in icteric subjects. There is also existed mention, that iodophthalein has also been used as a wound treatment, and for the treatment of intestinal catarrhs. But nowadays more modern drugs are being used.

Rose Bengal lactone is a polyhalogenated derivative of Fluorescein. Rose Bengal lactone is a dye compound described to produce cell membrane damage. Rose Bengal lactone and other Fluorescein derivatives are also described to modulate the function of ATP-sensitive K+ channels. Rose bengal lactone reacts readily with bases so treatment with triethylamine immediately yields the Rose Bengal salt.

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) is a chromogenic substrate for β-Galactosidase that forms an intense blue precipitate. X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect microorganisms which have β-Galactosidase activity (usually coliforms).

Rose Bengal lactone is a polyhalogenated derivative of Fluorescein. Rose Bengal lactone is a dye compound described to produce cell membrane damage. Rose Bengal lactone and other Fluorescein derivatives are also described to modulate the function of ATP-sensitive K+ channels. Rose bengal lactone reacts readily with bases so treatment with triethylamine immediately yields the Rose Bengal salt.