| Stereochemistry | ABSOLUTE |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.629 |
| Optical Activity | UNSPECIFIED |
| Defined Stereocenters | 5 / 5 |
| E/Z Centers | 0 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
OC[C@H]1O[C@@H](OC2=CNC3=CC=C(Br)C(Cl)=C23)[C@H](O)[C@@H](O)[C@H]1O
InChI
InChIKey=OPIFSICVWOWJMJ-AEOCFKNESA-N
InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.629 |
| Charge | 0 |
| Count |
MOL RATIO
1 MOL RATIO (average) |
| Stereochemistry | ABSOLUTE |
| Additional Stereochemistry | No |
| Defined Stereocenters | 5 / 5 |
| E/Z Centers | 0 |
| Optical Activity | UNSPECIFIED |
5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) is a chromogenic substrate for β-Galactosidase that forms an intense blue
precipitate. X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect microorganisms
which have β-Galactosidase activity (usually coliforms).
Originator
Approval Year
Targets
| Primary Target | Pharmacology | Condition | Potency |
|---|---|---|---|
Conditions
| Condition | Modality | Targets | Highest Phase | Product |
|---|---|---|---|---|
PubMed
Patents
Sample Use Guides
Protocol
Prepare a 20 mg/ml stock solution of 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) in N,Ndimethylformamide (DMF) or dimethylsulfoxide (DMSO).
Procedures
Blue/White Colony Screening
a) X-Gal included in agar: Add 5 ml of X-Gal stock solution and 5 ml of 0.1 M isopropyl-b-D-thiogalactoside (IPTG, BIO-37082) for each 1L of autoclaved media agar (e.g. LB agar) containing
appropriate antibiotics just before pouring. The media should be below 55 °C. Plate cells on cooled agar and incubate overnight at 37 °C.
b) X-Gal applied to top of agar: To a premade LB agar plate (e.g. prepared using LB agar), add 40 µl of X-Gal stock solution (at room temperature) and 4 µl of a 200 mg/ml solution of IPTG.
Spread solution over the entire surface of the plate. Incubate at 37 °C until the fluid is no longer visible. This may take several hours. Plate cells and incubate overnight at 37 °C.