TRENBOLONE ACETATE

Details

Stereochemistry	ABSOLUTE
Molecular Formula	C20H24O3
Molecular Weight	312.4028
Optical Activity	UNSPECIFIED
Defined Stereocenters	4 / 4
E/Z Centers	0
Charge	0

SHOW SMILES / InChI

Structure Search

SMILES

CC(=O)O[C@H]1CC[C@H]2[C@@H]3CCC4=CC(=O)CCC4=C3C=C[C@]12C

InChI

InChIKey=CMRJPMODSSEAPL-FYQPLNBISA-N

InChI=1S/C20H24O3/c1-12(21)23-19-8-7-18-17-5-3-13-11-14(22)4-6-15(13)16(17)9-10-20(18,19)2/h9-11,17-19H,3-8H2,1-2H3/t17-,18+,19+,20+/m1/s1

HIDE SMILES / InChI

Molecular Formula	C20H24O3
Molecular Weight	312.4028
Charge	0
Count

Stereochemistry	ABSOLUTE
Additional Stereochemistry	No
Defined Stereocenters	4 / 4
E/Z Centers	0
Optical Activity	UNSPECIFIED

Description
Sources: https://animaldrugsatfda.fda.gov/adafda/app/search/public/document/downloadFoi/221
Curator's Comment: description was created based on several sources, including: https://dailymed.nlm.nih.gov/dailymed/drugInfo.cfm?setid=de1d618f-7e27-4307-9330-0e1a2255ee95 http://www.wikidoc.org/index.php/Trenbolone

Trenbolone is an anabolic steroid. It is used on livestock to increase muscle growth and appetite. Trenbolone compounds have a binding affinity for the androgen receptor three times as high as that of testosterone. Once metabolized, the drugs have the effect of increasing nitrogen uptake by muscles, leading to an increase in the rate of protein synthesis. It also has the secondary effects of stimulating appetite, reducing the amount of fat being deposited in the body, and decreasing the rate of catabolism. Short-term side effects include insomnia, high blood pressure, increased aggression, night sweats, and libido.

CNS Activity

Originator

Approval Year

Targets

Primary Target	Pharmacology	Potency
Androgen Receptor 169 Target ID: CHEMBL1871 Sources: http://www.ncbi.nlm.nih.gov/pubmed/11252818	Binding Agent 1076	0.15 nM [Kd]
Progesterone receptor 61 Target ID: CHEMBL208 Sources: http://www.ncbi.nlm.nih.gov/pubmed/11252818	Binding Agent 1076
Glucocorticoid receptor 173 Target ID: CHEMBL2034 Sources: http://www.ncbi.nlm.nih.gov/pubmed/3486322	Binding Agent 1076	53.0 nM [Kd]

Conditions

Condition	Modality	Targets	Highest Phase	Product
Weight gain 4 Sources: http://www.accessdata.fda.gov/spl/data/d1f62758-6d8f-40df-8e6c-d7377f1fbd1e/d1f62758-6d8f-40df-8e6c-d7377f1fbd1e.xml	Primary		Approved 8583	Finaplix®-H Approved Use Increases rate of weight gain and improves feed efficiency. Launch Date 1987

Doses

Dose	Population	Adverse events
25 mg 1 times / day multiple, oral Overdose Dose: 25 mg, 1 times / day Route: oral Route: multiple Dose: 25 mg, 1 times / day Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/	healthy, ADULT Health Status: healthy Age Group: ADULT Sex: M Food Status: UNKNOWN Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/	Disc. AE: jaundice, pruritus... AEs leading to discontinuation/dose reduction: jaundice (1 pt) pruritus (1 pt) Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/

AEs

AE	Significance	Dose	Population
jaundice	1 pt Disc. AE	25 mg 1 times / day multiple, oral Overdose Dose: 25 mg, 1 times / day Route: oral Route: multiple Dose: 25 mg, 1 times / day Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/	healthy, ADULT Health Status: healthy Age Group: ADULT Sex: M Food Status: UNKNOWN Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/
pruritus	1 pt Disc. AE	25 mg 1 times / day multiple, oral Overdose Dose: 25 mg, 1 times / day Route: oral Route: multiple Dose: 25 mg, 1 times / day Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/	healthy, ADULT Health Status: healthy Age Group: ADULT Sex: M Food Status: UNKNOWN Sources: https://pubmed.ncbi.nlm.nih.gov/16436902/

PubMed

Title	Date	PubMed
Synthesis and characterization of new aromatic esters based on 4,16-pregnadiene-6,20-dione skeleton.	2010-12-08	21143843
Assessing the fate and transformation by-product potential of trenbolone during chlorination.	2010-11	20719355
Trenbolone causes irreversible masculinization of zebrafish at environmentally relevant concentrations.	2010-07-15	20363039
Tissue selectivity and potential clinical applications of trenbolone (17beta-hydroxyestra-4,9,11-trien-3-one): A potent anabolic steroid with reduced androgenic and estrogenic activity.	2010-06	20138077
Soil temperature and moisture effects on the persistence of synthetic androgen 17alpha-trenbolone, 17beta-trenbolone and trendione.	2010-05	20231030
OECD validation of phase-3 Hershberger assay using the stimulated weanling male rat in Korea.	2010-05	20082360
In vitro study of Organization for Economic Co-operation and Development (OECD) endocrine disruptor screening and testing methods- establishment of a recombinant rat androgen receptor (rrAR) binding assay.	2010-04	20371976
Generic sample preparation combined with high-resolution liquid chromatography-time-of-flight mass spectrometry for unification of urine screening in doping-control laboratories.	2010-04	20155493
Highly active human pharmaceuticals in aquatic systems: A concept for their identification based on their mode of action.	2010-02-18	20053463
Sex differences in the uptake and disposition of perfluorooctanoic acid in fathead minnows after oral dosing.	2010-01-01	19961202
Hormonal growth promoting agents in food producing animals.	2010	20020373
Gonadal alterations in male eelpout (Zoarces viviparus) exposed to ethinylestradiol and trenbolone separately or in combination.	2010	19864016
Stereoselective sorption by agricultural soils and liquid-liquid partitioning of trenbolone (17alpha and 17beta) and trendione.	2009-12-01	19943653
Confirmatory method for the determination of nandrolone and trenbolone in urine samples using immunoaffinity cleanup and liquid chromatography-tandem mass spectrometry.	2009-11-13	19447393
Is the gonadotropin releasing hormone system vulnerable to endocrine disruption in birds?	2009-09-01	19457435
Analysis of anabolic steroids in hair: time courses in guinea pigs.	2009-09	19397917
Synthesis and SAR study of novel pseudo-steroids as potent and selective progesterone receptor antagonists.	2009-07-15	19217285
Expression signatures for a model androgen and antiandrogen in the fathead minnow (Pimephales promelas) ovary.	2009-04-01	19452925
Determination of trenbolone residual in bovine liver by liquid chromatography-mass spectrometry.	2009-04	18953471
Doping control analysis of trenbolone and related compounds using liquid chromatography-tandem mass spectrometry.	2009-03	19007801
The watershed as a conceptual framework for the study of environmental and human health.	2009-02-18	20508751
Identification of hormone esters in injection site in muscle tissues by LC/MS/MS.	2008-12	19680861
The development of multiple drug use among anabolic-androgenic steroid users: six subjective case reports.	2008-11-28	19040748
[Determination of eleven steroid hormones in animal muscle tissues and eggs using ultra-performance liquid chromatography-tandem mass spectrometry].	2008-11	19253550
Chemical contaminants in feedlot wastes: concentrations, effects and attenuation.	2008-08	18055014
[Determination of three anabolic steroids by liquid chromatography-mass spectrometry].	2008-07	18959243
Effect of parental exposure to trenbolone and the brominated flame retardant BDE-47 on fertility in rainbow trout (Oncorhynchus mykiss).	2008-07	18397801
Trace analysis of androgens and progestogens in environmental waters by ultra-performance liquid chromatography-electrospray tandem mass spectrometry.	2008-06-27	18502434
Distribution of trenbolone residues in liver and various muscle groups of heifers that received multiple implants at the recommended site of application.	2008-06-24	18567315
Comparison of estrogen-responsive plasma protein biomarkers and reproductive endpoints in sheepshead minnows exposed to 17beta-trenbolone.	2008-06-23	18495259
Confirmatory analysis of Trenbolone using accurate mass measurement with LC/TOF-MS.	2008-06-16	18501249
Degradation of synthetic androgens 17alpha- and 17beta-trenbolone and trendione in agricultural soils.	2008-05-15	18546691
Induction of micronuclei in V79 cells by the anabolic doping steroids tetrahydrogestrinone and trenbolone.	2008-04	17764006
The detection and assessment of the aneugenic potential of selected oestrogens, progestins and androgens using the in vitro cytokinesis blocked micronucleus assay.	2008-03-12	18069048
Intercalibration exercise using a stickleback endocrine disrupter screening assay.	2008-02	18348632
Screening of in vitro synthesised metabolites of 4,9,11-trien-3-one steroids by liquid chromatography mass spectrometry.	2008	18708698
Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds.	2007-12-01	17822730
In utero exposure to the environmental androgen trenbolone masculinizes female Sprague-Dawley rats.	2007-11-01	17931805
Determination of anabolic steroids in muscle tissue by liquid chromatography-tandem mass spectrometry.	2007-10-17	17887801
A league of their own: demographics, motivations and patterns of use of 1,955 male adult non-medical anabolic steroid users in the United States.	2007-10-11	17931410
Evaluation of the rodent Hershberger bioassay: testing of coded chemicals and supplementary molecular-biological and biochemical investigations.	2007-09-24	17688994
Rapid screening of doping agents in human urine by vacuum MALDI-linear ion trap mass spectrometry.	2007-08-01	17602668
Analysis of synthetic 19-norsteroids trenbolone, tetrahydrogestrinone and gestrinone by gas chromatography-mass spectrometry.	2007-05-25	16963059
Determination of synthetic hormones in animal urine by high-performance liquid chromatography/mass spectrometry.	2007-05-04	17474533
The OECD program to validate the rat Hershberger bioassay to screen compounds for in vivo androgen and antiandrogen responses: phase 2 dose-response studies.	2007-05	17520051
Immunotoxicity of trenbolone acetate in Japanese quail.	2007-01	17162502
Reproductive toxicity of trenbolone acetate in embryonically exposed Japanese quail.	2007-01	16989888
Doping control analysis in human urine by liquid chromatography-electrospray ionization ion trap mass spectrometry for the Olympic Games Athens 2004: determination of corticosteroids and quantification of ephedrines, salbutamol and morphine.	2006-07-28	17723530
Application of ecotoxicogenomics for studying endocrine disruption in vertebrates and invertebrates.	2006-04	16818254
Rapid test by liquid chromatography/tandem mass spectrometry to evaluate equine urine reactivity towards 17beta-OH steroids.	2006	16862626

Patents

Sample Use Guides

In Vivo Use Guide

One implant containing 200 mg trenbolone acetate is administered to each animal. The implant is placed under the skin on the posterior aspect of the ear by means of a special implanter.

Route of Administration: Other

In Vitro Use Guide

Trenbolone was a high-affinity ligand for the androgen receptor (AR), with an IC(50) of about 4 nM in rat ventral prostate cytosol and about 33 nM in cells transfected with the human AR.

Substance Class	Chemical Created by `admin` on `Mon Mar 31 18:23:29 GMT 2025` Edited by `admin` on `Mon Mar 31 18:23:29 GMT 2025`
Record UNII	RUD5Y4SV0S
Record Status	`Validated (UNII)`
Record Version

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Name

Type

Language

TRENBOLONE ACETATE

Official Name

English

TRENBOLONE ACETATE CIII

Preferred Name

English

TRENBOLONE ACETATE CIII [USP-RS]

Common Name

English

TRENBOLONE ACETATE [MI]

Common Name

English

TRENBOLONE ACETATE [USAN]

Common Name

English

TRENBOLONE ACETATE [MART.]

Common Name

English

ESTRA-4,9,11-TRIEN-3-ONE, 17-(ACETYLOXY)-, (17.BETA.)-

Systematic Name

English

RU-1697

Code

English

17?-Hydroxyestra-4,9,11-trien-3-one, acetate

Common Name

English

TRENBOLONE ACETATE [USP MONOGRAPH]

Common Name

English

Trenbolone acetate [WHO-DD]

Common Name

English

TRENBOLONE ACETATE [GREEN BOOK]

Common Name

English

TRENBOLONE ACETATE [USP IMPURITY]

Common Name

English

Classification Tree Code System Code

CFR

21 CFR 522.2476