A dose of 355-385 MBq of [18F]FMAU was intravenously infused into patients over 60s.

Breast cancer cells were transduced with herpes simplex virus 1 thymidine kinase. Logarithmically growing cells were incubated with about 111 –148 KBq (3 - 4 uCi) of [18F]FMAU. Following incubation and removal of media, the cells were washed with phosphate-buffered saline (PBS, 3x10 mL) and treated with 0.3N perchloric acid. The acidic mixtures were cooled to 4°C for 10 min, centrifuged for 1 min at 14,000 rpm and the supernatant was separated. The cell pellets were washed twice with PCA (1 mL), centrifuged, and the acid soluble fractions, washings, and cell pellets were treated with complete counting scintillation cocktail. Radioactivity was counted in a liquid scintillation counter.