in rats (microinjected into the lateral brain ventricle): (α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) produced generalized myoclonic seizures, short lasting hypoactivity followed by hyperactivity and hyperthermia when low doses were injected (0.25-1.0 microgram). When AMPA was injected at higher doses (1.5-5.0 microgram) it produced generalized myoclonic seizures, a hypoactive phase and hypothermia rapidly followed by hyperthermia

Binding of [3H]AMPA (α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) to rat brain membranes was investigated. Computer-aided Scatchard analysis of the binding data, as determined by using L-glutamic acid (L-GLU) to define nonspecific binding, suggested the presence of two independent binding sites, with KDS of 9 and 2440 nM, respectively. Additional freezing, thawing and washing sequences gave membranes with only one binding site, with a KD of 278 nM. [3H]AMPA binding exhibited the highest level in striatal membranes.