NDT 9513727 activity was evaluated in C5a-Induced Neutropenia in Cynomolgus Macaque. Two males and two females weighing 3 to 4 kg were used in each experiment, except in the 75 mg/kg study (two males, one female). Animals were given ad libitum access to water during the experiments, but no food was provided during the period of 8 h before to approximately 5 h after drug administration. NDT 9513727 was suspended in 0.5% methylcellulose/0.1% triacetin in water and stirred overnight in the dark before administration. Drug solution or vehicle was administered at doses 5, 25 and 75 mg/kg by nasogastric gavage in a volume of 10 ml/kg body weight.

U937 cells were incubated with varying concentrations of NDT 9513727, ranging from 0.1 nM to 4 mkM, for 1 h before the addition of 0.3 nM hC5a (an EC50 concentration). The fluorescence response, upon the subsequent addition of the EC50 concentration of hC5a, was then monitored using an FLIPR.