In Hot - plate test rats were treated with QA (Quillaic acid) at doses 0.5, 1.0, 1.5, 2.0 mg/kg (i.p.) In Tail flick test rats were treated with QA (Quillaic acid) at doses 2.5, 7.5 and 22.5 mg/kg (Topical)

MDA-MB-231 human breast cancer and PC-3 human prostate cancer cell lines were used for activity evaluation. The antiproliferative activity of extracts was evaluated using the AbD serotec alamarBlue assay. Cells were seeded at 2500 cells per well in 96-well tissue culture plates. After 18–24 h, cells were exposed to various concentrations of saponins with the final concentration of DMSO not exceeding 0.1%. Cells were incubated for 48 h, with alamar-Blue dye solution added to each well to make up 10% of the final volume for the final six to eight hours. Fluorescence was measured at an excitation of 560 nm and emission of 590 nm on a Perkin Elmer 1420 VICTOR3V Multilabel Counter.