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Details

Stereochemistry ACHIRAL
Molecular Formula C26H33N3
Molecular Weight 387.5603
Optical Activity NONE
Defined Stereocenters 0 / 1
E/Z Centers 0
Charge 2

SHOW SMILES / InChI
Structure of METHYL GREEN CATION

SMILES

CN(C)C1=CC=C(C=C1)C(C2=CC=C(C=C2)[N+](C)(C)C)=C3C=CC(C=C3)=[N+](C)C

InChI

InChIKey=CKTYULMXIIQZGG-UHFFFAOYSA-N
InChI=1S/C26H33N3/c1-27(2)23-14-8-20(9-15-23)26(21-10-16-24(17-11-21)28(3)4)22-12-18-25(19-13-22)29(5,6)7/h8-19H,1-7H3/q+2

HIDE SMILES / InChI

Molecular Formula C26H33N3
Molecular Weight 387.5603
Charge 2
Count
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 1
E/Z Centers 0
Optical Activity NONE

Description
Sources: DOI: 10.1201/9780849382192.ch11, Sabnis R.W., 'Handbook of Acid-Base Indicators' (2007), p. 149

A DNA stain used as a tracking dye in acid buffers for electrophoresis. Methyl Green was used as a topical antiseptic, however, it was withdrawn due to unwanted effects. The traditional Methyl Green has not manufactured for decades. The dye sold as Methyl Green is more accurately described as Ethyl Green. Both, Methyl Green and Ethyl Green are sold as double zinc salts.

Approval Year

PubMed

PubMed

TitleDatePubMed
Methyl green-pyronin stain for the diagnosis of trachoma.
1969-04
Interaction of methyl green with deoxyribonucleic acid as observed by a new method of using hydrogen peroxide.
1963-02
On the nature of the deoxyribonucleic acid-methyl green reaction.
1958-09-25
Methyl-green-pyronin as a differential nucleic acid stain for peripheral blood smears.
1956-12
Spectrophotometry of dyes. I. Methyl green. II. Pyronin.
1953-11
Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.
1950-11
The Nature of the Specificity of Methyl Green for Chromatin.
1949-02
Patents

Sample Use Guides

Methyl green inhibited B.aerogenis, B.coli, B.typhosus, with the inhibiting dilution of 1200 and B.proteus with he inhibiting dilution of 2700.
Substance Class Chemical
Created
by admin
on Wed Apr 02 05:37:12 GMT 2025
Edited
by admin
on Wed Apr 02 05:37:12 GMT 2025
Record UNII
105IDS5C5F
Record Status Validated (UNII)
Record Version
  • Download
Name Type Language
4-((4-(DIMETHYLAMINO)PHENYL)(4-(DIMETHYLIMINIO)-2,5-CYCLOHEXADIEN-1-YLIDENE)METHYL)-N,N,N-TRIMETHYLBENZENAMINIUM
Preferred Name English
METHYL GREEN CATION
Common Name English
AMMONIUM, (.ALPHA.-(P-(DIMETHYLAMINO)PHENYL)-.ALPHA.-(4-(DIMETHYLIMINIO)-2,5-CYCLOHEXADIEN-1-YLIDENE)-P-TOLYL)TRIMETHYL-
Systematic Name English
BENZENAMINIUM, 4-((4-(DIMETHYLAMINO)PHENYL)(4-(DIMETHYLIMINIO)-2,5-CYCLOHEXADIEN-1-YLIDENE)METHYL)-N,N,N-TRIMETHYL-
Systematic Name English
Code System Code Type Description
CAS
7438-47-3
Created by admin on Wed Apr 02 05:37:12 GMT 2025 , Edited by admin on Wed Apr 02 05:37:12 GMT 2025
PRIMARY
FDA UNII
105IDS5C5F
Created by admin on Wed Apr 02 05:37:12 GMT 2025 , Edited by admin on Wed Apr 02 05:37:12 GMT 2025
PRIMARY
CHEBI
87650
Created by admin on Wed Apr 02 05:37:12 GMT 2025 , Edited by admin on Wed Apr 02 05:37:12 GMT 2025
PRIMARY
PUBCHEM
6728
Created by admin on Wed Apr 02 05:37:12 GMT 2025 , Edited by admin on Wed Apr 02 05:37:12 GMT 2025
PRIMARY
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