Trolox (6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) is a synthetic Vitamin E analog exerting antioxidant properties. Trolox is widely used for estimation of antioxidant capacity in the assay based on electron transfer reaction. Trolox-equivalent antioxidant capacity (TEAC) assay is based on the suppression of the absorbance of radical cations of 2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonate) (ABTS) by antioxidants in the test sample when ABTS incubates with a peroxidase (metmyoglobin) and hydrogen peroxide.