Oftasceine is a fluorescent dye or luminescent agent. It was recommended for the use as an effective agent for the blood-retinal barrier integrity studies. It was used to detect drug interactions with multidrug resistance proteins because it is an effective substrate of the multidrug resistance transporter 1P-glycoprotein and the multidrug resistance-related protein. The Oftasceine assay can be used as a quantitative, standardized, inexpensive screening test in a routine clinical laboratory setting. The assay detects both P-glycoprotein and multidrug resistance-associated protein activities, and identifies acute myeloid leukaemia patients with unfavorable therapy responses. It is used in ophthalmic solutions as a staining agent when fitting hydrogel contact lens. The use of a fluorexon dye-impregnated strip (pre-wetted with preservative-free saline) yielded comparable results with a fluorescein strip for assessments of tear film stability based on tear film break-up time.