In the mouse model of stress-induced hyperthermia, CBiPES was administered subcutaneously at 100 mg/kg.

mGluR2 potentiation was tested in cell lines that expressed each of the eight human mGlu receptor, mGlu1a, mGlu2, mGlu3, mGlu4a, mGlu5a, mGlu6, mGlu7a, or mGlu8a, in an AV-12 (ATCC-CRL9595) cell background that also stably expresses the rat glutamate aspartate transporter (EAAT-1 or GLAST). With the group II and III receptors (mGlu2/3/4/6/7/8), the promiscuous G protein Gα15 was also stably expressed. This allowed receptor activation to stimulate phospholipase C, increasing inositol triphosphate formation, and subsequently a transient rise of intracellular calcium. Quantitation utilized to be determined using a calcium-sensitive dye and a Fluorometric Imaging Plate Reader (FLIPR). Treatment with CBiPES potentiated glutamate-induced increase in intracellular calcium concentration with IC50 of 92.8 nM