In turkeys has been used as a feed additive at a level of 50 - 75 mg/kg (ppm) subject of a 5 day withdrawal period prior to slaughter.

Histomonas meleagridis cultures were isolated from the droppings of chickens orally infected by Heterakis gallinarum, which subsequently developed Histomoniasis. Parasites and accompanying bacterial flora were cultured anaerobically at 39 deg-C on Stepkowski media. The accompanying flora, which are essential to in vitro culture of the parasite, were isolated from the cecum of infected birds. Nifursol was dissolved in dimethyl sulfoxide at 8 mg/mL. H. meleagridis cultures (10^6 parasites /mL) were incubated anaerobically for 24-hours at 39 deg-C. Nifursol was assayed in a range of concentrations between 12.5 - 400 micro-g/mL. The viability of cultures was determined at 6, 12, 24, and 48 hours. The minimum lethal concentration (MLC) was defined as the lowest drug concentration in which no live organisms were observed. Nifursol was effective against H. meleagridis even though it did not kill all parasites after 24, 48 and 72 hours. However, there was no time effect, regardless of concentration; therefore, Nifursol may be regarded as a growth inhibitor.