New Zealand white male rabbits were treated with 10 mg/kg gossypetin of 10 weeks

A cell viability test of GP (Gossypetin) was performed on cultured human lymphocytes to detect the maximal non-toxic concentration according to the manufacturer’s protocol. A total of 1 × 10^4 cells were seeded on each well of a 96-well plate and cultured for 24 hours. The cells were treated with different concentrations of CP (1mM), GP (25, 50 and 100 µM) and CP+GP (25, 50 and 100 µM) combinations in a serum-free medium for 24 and 48 hours. Gossypetin concentrations were selected according to previous studies. At the end of the incubation process/time, the medium containing compound was exchanged with a fresh RPMI1640 medium, and 10 μL of the reconstitute MTT mixture kit reagent were added to each well. After culturing for 4 hours, the absorbance of the sample was measured by using a microtiter plate reader (Bio-TEK) at 590 nm.