Mice were treated with JWH-210 (10 mg/kg, 3 days, i.p.)

Splenocytes were cultured from naïve mice as mentioned previously. The cells were seeded in 96-well plates (5 × 10^5 cells/ well) with 100 μL media. After seeding single cell suspensions, the plates were incubated for 6 h in 95% air:5% CO2 using standard culture methods. To confirm cell viability by synthetic cannabinoids, the splenocytes were treated with synthetic cannabinoids (0, 1, 10, and 100 μM) for 16 h. To observe relationship between JWH-210 and CB receptors, the splenocytes were treated with media or JWH-210 or JWH-210/CB receptor antagonist (rimonabant or AM630) for 4 h.