Fifteen healthy male volunteers were injected with 0.09 - 0.65 μg [11C]SCH-23390. The injections had a total radioactivity of 248 - 417 MBq and radioactivity per kg body weight of 3.16 and 4.79 MBq/kg. PET imaging data was acquired over 51 minutes in a series of consecutive thirteen minute time frames with durations of 3×60 s, 4×180 s and 6×360 s. Investigators were interested in comparing volumetric and surface-based registration and smoothing methods and found that surface-based methods yielded higher BP-ND values, lower coefficient of variation, less bias, better reliability and more precise estimates of parametric binding; therefore providing superior performance to volumetric approaches for voxelwise analysis of PET data

Rat brain striatal slices were preincubated with [3H]GABA and superfused in the presence of the GABA transport inhibitor nipecotic and GABA aminotransferase inhibitor aminooxyacetic acids. GABA efflux was estimated by monitoring the efflux of [3H]GABA. The overflow of GABA-evoked by electrical field stimulation was blocked by 0.5 - 10 microM of SCH 23390