Patients hospitalized due to ICP received epomediol 900 mg/day (n = 7), or 1,200 mg/day (n = 4) orally, during 15 days.

Hepatocytes were incubated at different times (15-60 sec. and 5-30 min.) in the presence of different concentrations of labeled and unlabeled taurocholate ( 1 : 9) to determine ['H]-taurocholate uptake. The incubation medium was then rapidly removed and the dishes (3 for each experimental group) were immediately washed with cold saline. In fact, no measurable efflux of bile acids from isolated hepatocytes has been proved to exist at 0C. Finally. 1.5 ml NaOH 0.33 N was added to each dish and after 30 min. 0.5 ml and 0.2 ml of the cellular lysate were taken to count radioactivity and protein content respectively.