Enniatin B is a cyclic hexadepsipeptide it contains three N-methyl-Lvaline and three a-hydroxy-D-isovaleric molecules. It is generated by multiple Fusarium strains. It has ionophoric, antibiotic and insecticidal activity. A mixture of Enniatins A, A1, B and B1 (called fusafungine) was originally patented as local antibiotic for the topically treatment of nose and throat infections. Enniatin B is a mycotoxin contaminant found in vegetables, cereals and coffee. Enniatin B did not show genotoxic activity but demonstrated cytotoxicity at low micromolar concentrations. The observed activities included the specific inhibition of acyl-CoA cholesterol acyltransferase, depolarization of mitochondria, inhibition of osteoclastic bone resorption, and induction of apoptosis in cancer cells as well as the interaction with ATP-binding cassette transporters such as P-glycoprotein. Enniatin B demonstrated synergistic activity against cervical cancer in vitro and in vivo.