ranges for Sodium Lauryl Sulfoacetate 0.6–21%

10mL of pooled blood sample was collected from 10 chickens and onefold was diluted with PBS. Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation on 14mL Ficoll-paque lymphocyte separation medium at 400 g for 15 minutes and resuspended (1x10^6 cells/mL) in RPMI 1640 medium supplemented with 10% FCS, 100 IU/mL penicillin, and 100 μg/mL streptomycin. Lymphocyte proliferation was measured using MTT method. 100 μL of the cell suspension was dispersed into each well of 96-well plates and then 62.5, 125, 250 or 500 μg/mL SLS was added (n = 12). After incubation at 39.5◦C, 5% CO2 for 44 hours, 10 μL MTT (5mg/mL) was added into each well and the incubation was continued for 4 hours. Then 100 μL of DMSO was added and incubation was continued for additional 24 hours before measurement for OD570 values using an ELISA reader (Bio-Tek Instruments, VT). The cell suspension without SLS treatment and with 5 μg/mL Con A was used as the negative and positive control, respectively.