Unknown

Cytotoxic effects of FA-AB (Flavaspidic acid AB) were evaluated by the MTT (3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide) assay. Marc-145 cells or PAMs in a 96-well plate were cultured in 100 ll DMEM or RPMI 1640 containing 0, 10, 20, 30, 40, 50, 60, 70, or 80 lg/ml FA-AB for 48 h at 37 _C. Next, the culture medium was replaced with fresh medium containing 20 mkl of 5 mg/ml MTT after washing three times with PBS, and cells were further cultured for 4 h at 37 C. Cells were then washed carefully and 150 mkl DMSO was added per well to dissolve the crystals for 10 min. The resulting absorbance of each well was recorded at 490 nm using a plate reader.