A fatal intoxication: dosage range of 1.5–3.0 mg with duration time of 12–24 h. At the highest ingested dose (2.4 mg)

5-HT2A and 5-HT2C receptors HEK cells expressing the human 5-HT2A receptor (HEK-5-HT2A cells) or the human 5-HT2C receptor (HEK-5-HT2C cells) were used. In the 5-HT2A [3H]AA release assay, 2,5-Dimethoxy-4-chloroamphetamine (DOC) and 5-HT were agonists with similar efficacies while potencies differed significantly. DOC were very potent with EC50 values that did not differ from serotonin and LSD. In HEK-h5-HT2C cells, the phenethylamines were tested for their affinities for the [125I]DOI binding site and effects on 5-HT2C-mediated IP-1 turnover. In the [125I]DOI binding assay, there were significant differences in affinities DOC, serotonin and LSD had similar, low nanomolar, affinities.