2,4-Diacetylphloroglucinol (DAPG) is a secondary metabolite of the P. Fluorescens strain CHAo which inhibits bacteria, fungi, and plants and suppresses pathogens. 2,4-Diacetylphlo roglucinol also displays antiviral activity against DNA and RNA viruses with envelopes. 2,4-Diacetylphloroglucinol exhibited a broad-spectrum of anti-leukemic, anti-lung, and anti-breast cancer properties. The anticancer and antimetastatic activities of DAPG were mediated by the inhibition of ROS, NF-κB, Bcl-2, MMP-2, VEGF and primary inflammatory mediators such as TNF-α, IL-6, IL-1β and NO. The DAPG induced apoptosis in cancer cells by intrinsic and extrinsic pathways via the release of cytochrome-C, upregulation of Bax and the activation of caspases and also, exhibited anti-inflammatory activity by the inhibition of LPS-inflammed cell proliferation of macrophage (Raw 264.7), monocytic cells (THP-1) and peripheral blood mononuclear cells (PBMCs). Results further confirmed that the DAPG inhibited the primary inflammatory mediators in cancer cells and inflammed immune cells through the down regulation of NF-κB.