Zucker fatty rats was treatment with K579 at a doses 0.1, 0.3 or 1mg/kg

GLUTag cells were seeded onto poly-D lysine coated 96 well plates at 8000 cells/well and grown for approximately 48 h. Cells were incubated in assay buffer containing test ligand for1 h at 37 °C. Cells were then washed by four exchanges of fresh buffer, before exposure to buffer containing the diepetidyl peptidase IV inhibitor K-579(100nM)7test compoundfor2hat37 °C. Supernatant was then removed and GLP-1 concentration measured by the 1 concentration measured by the Millipore ELISA kit, as per the manufacturers instructions. Fluorescencewas measured at abs/emof355/460nm using a TecanSafire, and samples compared to a standard curve.