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Details

Stereochemistry ABSOLUTE
Molecular Formula C28H28N4O3
Molecular Weight 468.5469
Optical Activity UNSPECIFIED
Defined Stereocenters 1 / 1
E/Z Centers 0
Charge 0

SHOW SMILES / InChI
Structure of RUBOXISTAURIN

SMILES

CN(C)C[C@@H]1CCN2C=C(C3=C2C=CC=C3)C4=C(C(=O)NC4=O)C5=CN(CCO1)C6=C5C=CC=C6

InChI

InChIKey=ZCBUQCWBWNUWSU-SFHVURJKSA-N
InChI=1S/C28H28N4O3/c1-30(2)15-18-11-12-31-16-21(19-7-3-5-9-23(19)31)25-26(28(34)29-27(25)33)22-17-32(13-14-35-18)24-10-6-4-8-20(22)24/h3-10,16-18H,11-15H2,1-2H3,(H,29,33,34)/t18-/m0/s1

HIDE SMILES / InChI

Molecular Formula C28H28N4O3
Molecular Weight 468.5469
Charge 0
Count
MOL RATIO 1 MOL RATIO (average)
Stereochemistry ABSOLUTE
Additional Stereochemistry No
Defined Stereocenters 1 / 1
E/Z Centers 0
Optical Activity UNSPECIFIED

Description

Ruboxistaurin is an orally bioavailable, selective, potent inhibitor of protein kinase C β developed for treating diabetic retinopathy. In vitro and in vivo non-clinical models have demonstrated that Ruboxistaurin decreases PKC β activity and ameliorates many of the effects of PKC β on pathologic processes in the retina. Ruboxistaurin prevents the slowing of retinal blood flow that is observed by fluorescein video angiography in the eyes of diabetic rats. It is also reported to cause regression of retinal neovascularization produced by laser-induced major branch vein occlusions in a porcine model. Ruboxistaurin positively affected the diabetes-induced retinal blood flow abnormalities in a Phase Ib study in diabetic patients. Ruboxistaurin is in phase III clinical trials for the treatment of diabetic nephropathy and diabetic macular edema. Eli Lilly had submitted Ruboxistaurin for approval in the US and the EU; however, the company subsequently discontinued development as it was unable to demonstrate sufficient efficacy

Originator

Approval Year

Targets

Primary TargetPharmacologyConditionPotency
5.9 nM [IC50]
360.0 nM [IC50]
300.0 nM [IC50]
52.0 nM [IC50]

Conditions

ConditionModalityTargetsHighest PhaseProduct
Primary
Unknown
Primary
Unknown
Primary
Unknown
Primary
Unknown
Primary
Unknown

Cmax

ValueDoseCo-administeredAnalytePopulation
204 nM
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens
200 nM
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens

AUC

ValueDoseCo-administeredAnalytePopulation
1410 nM × h
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens
1364 nM × h
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens

T1/2

ValueDoseCo-administeredAnalytePopulation
3.54 h
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens
13.8 h
64 mg single, oral
RUBOXISTAURIN plasma
Homo sapiens

PubMed

Patents

Sample Use Guides

In Vivo Use Guide
32 mg/day
Route of Administration: Oral
In Vitro Use Guide
HUVECs co-cultured with fibroblasts were cultivated in the presence or absence of various concentrations of ruboxistaurin (0.1 and 1 mkM) plus VEGF (10 ng/ml) at days 1, 4, 7 and 9. The ruboxistaurin was dissolved in dimethyl sulfoxide (DMSO, final concentration of DMSO was 0.1%). DMSO (0.1%) was added to the non-drug control (control group). At day 11, cells were fixed in 70% ethanol. The cells were incubated with diluted primary antibody (mouse anti-human CD31, 1 : 4000) for 1 h at 37 C, and with the secondary antibody (goat anti-mouse IgG alkaline phosphataseconjugated antibody, 1 : 500) for 1 h at 37 C. Visualization was achieved using 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium.
Substance Class Chemical
Record UNII
721809WQCP
Record Status Validated (UNII)
Record Version