Male Sprague-Dawley rats were induced to inflammatory paw pain by subcutaneous injection of a 0.1 mL 1% suspension of carrageenan in saline. Prophylactic treatment with CJ-13610 (1 h before carrageenan) dose-dependently reversed mechanical hyperalgesia by 38 + 7%, 46 + 3% and 90 + 4% with 1, 10 and 30 mg/kg respectively. The CJ-13610 dose was delivered as a 1 mL oral gavage in a vehicle containing 0.5% methylcellulose and 0.025% Tween-20.

Human blood was collected in 10 mL heparinized tubes and pooled and dispensed into 384 well experimental plates. Varying concentrations of CJ13610 were dissolved in DMSO, and 2 micro-L was added to each well. CJ13610 was preincubated with blood at room temperature for 10 minutes, followed by stimulation with 40 micro-M calcium ionophore and 30 micro-M arachidonic acid dissolved in 60% ethanol (intended to stimulate maximum leukotriene production). After 15 minute incubation at 37 deg-C blood was centrifuged and leukotriene levels measured from the supernatant. CJ13610 was found to have an IC50 of 0.57 micro-M against the production of leukotrienes.