U.S. Department of Health & Human Services Divider Arrow National Institutes of Health Divider Arrow NCATS

Details

Stereochemistry ACHIRAL
Molecular Formula C16H20N2
Molecular Weight 240.3434
Optical Activity NONE
Defined Stereocenters 0 / 0
E/Z Centers 0
Charge 0

SHOW SMILES / InChI
Structure of 3,3',5,5'-TETRAMETHYLBENZIDINE

SMILES

CC1=CC(=CC(C)=C1N)C2=CC(C)=C(N)C(C)=C2

InChI

InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

HIDE SMILES / InChI

Molecular Formula C16H20N2
Molecular Weight 240.3434
Charge 0
Count
MOL RATIO 1 MOL RATIO (average)
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 0
E/Z Centers 0
Optical Activity NONE

Description

3,3',5,5'-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase. TMB is converted to a blue colored diimine, which can be registered on a spectrophotometer at a wavelength of 650 nM. TMB is commonly used in techniques such as ELISA and immunohistochemistry.

Originator

Approval Year

Targets

Primary TargetPharmacologyConditionPotency

Conditions

ConditionModalityTargetsHighest PhaseProduct

PubMed

Sample Use Guides

In Vivo Use Guide
Unknown
Route of Administration: Unknown
In Vitro Use Guide
The incubation mixture contained 10 uM of 3,5,3',5'-tetramethylbenzidine and 15 uM of hydrogen peroxide in acetate buffer, pH 5.0. At zero time, horseradish peroxidase was added (10 ng/ml).
Substance Class Chemical
Record UNII
3B3T5CB8EO
Record Status Validated (UNII)
Record Version