3,3',5,5'-TETRAMETHYLBENZIDINE

Details

Stereochemistry	ACHIRAL
Molecular Formula	C16H20N2
Molecular Weight	240.3434
Optical Activity	NONE
Defined Stereocenters	0 / 0
E/Z Centers	0
Charge	0

SHOW SMILES / InChI

Structure of 3,3',5,5'-TETRAMETHYLBENZIDINE

SMILES

CC1=CC(=CC(C)=C1N)C2=CC(C)=C(N)C(C)=C2

InChI

InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

HIDE SMILES / InChI

Molecular Formula	C16H20N2
Molecular Weight	240.3434
Charge	0
Count	MOL RATIO 1 MOL RATIO (average)

Stereochemistry	ACHIRAL
Additional Stereochemistry	No
Defined Stereocenters	0 / 0
E/Z Centers	0
Optical Activity	NONE

Description

3,3',5,5'-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase. TMB is converted to a blue colored diimine, which can be registered on a spectrophotometer at a wavelength of 650 nM. TMB is commonly used in techniques such as ELISA and immunohistochemistry.

Originator

Approval Year

Targets

Primary Target	Pharmacology	Condition	Potency
Peroxidase C1A 3	Substrate 661

Conditions

Condition	Modality	Targets	Highest Phase	Product
Unknown 2,578

PubMed

Show entries

Title	Date	PubMed
Motoneurons of the lateral and medial rectus extraocular muscles in squirrel monkey and cat.	2001	11173806
Reaction between albumen and 3,3',5,5'-tetramethylbenzidine as a method to evaluate egg freshness.	2001 Aug	11513622
Comparison of wheat germ agglutinin-horseradish peroxidase and biotinylated dextran for anterograde tracing of corticospinal tract following spinal cord injury.	2001 Aug 30	11513942
Peroxidase-catalyzed co-oxidation of 3,3',5,5'-tetramethylbenzidine with 2-amino-4-nitrophenol, 4,4'-dihydroxydiphenylsulfone, and their polydisulfides in aqueous and micellar media.	2001 Jun	11421808
Localization of intracellular lipid hydroperoxides using the tetramethylbenzidine reaction for transmission electron microscopy.	2002	12152190

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Patents

Sample Use Guides

In Vivo Use Guide

Unknown

Route of Administration: Unknown

In Vitro Use Guide

The incubation mixture contained 10 uM of 3,5,3',5'-tetramethylbenzidine and 15 uM of hydrogen peroxide in acetate buffer, pH 5.0. At zero time, horseradish peroxidase was added (10 ng/ml).

Substance Class	Chemical
Record UNII	3B3T5CB8EO
Record Status	`Validated (UNII)`
Record Version

Show entries

Name

Type

Language

3,3',5,5'-TETRAMETHYLBENZIDINE

Systematic Name

English

COLORBURST BLUE

Brand Name

English

3,3',5,5'-TETRAMETHYLBENZIDINE [HSDB]

Common Name

English

3,3',5,5'-TETRAMETHYL(1,1'-BIPHENYL)-4,4'-DIAMINE

Systematic Name

English

POTASSIUM-BLUE MAX

Brand Name

English

Showing 1 to 5 of 9 entries

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Show entries

Code System

Code

Type

Description

WIKIPEDIA

3,3',5,5'-Tetramethylbenzidine

PRIMARY

HSDB

4331

PRIMARY

FDA UNII

3B3T5CB8EO

PRIMARY

MERCK INDEX

m11776

PRIMARY

PUBCHEM

41206

PRIMARY

Showing 1 to 5 of 8 entries

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