3,3',5,5'-TETRAMETHYLBENZIDINE

Details

Stereochemistry	ACHIRAL
Molecular Formula	C16H20N2
Molecular Weight	240.3434
Optical Activity	NONE
Defined Stereocenters	0 / 0
E/Z Centers	0
Charge	0

SHOW SMILES / InChI

Structure of 3,3',5,5'-TETRAMETHYLBENZIDINE

SMILES

CC1=CC(=CC(C)=C1N)C2=CC(C)=C(N)C(C)=C2

InChI

InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

HIDE SMILES / InChI

Molecular Formula	C16H20N2
Molecular Weight	240.3434
Charge	0
Count	MOL RATIO 1 MOL RATIO (average)

Stereochemistry	ACHIRAL
Additional Stereochemistry	No
Defined Stereocenters	0 / 0
E/Z Centers	0
Optical Activity	NONE

Description

3,3',5,5'-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase. TMB is converted to a blue colored diimine, which can be registered on a spectrophotometer at a wavelength of 650 nM. TMB is commonly used in techniques such as ELISA and immunohistochemistry.

Originator

Approval Year

Targets

Primary Target	Pharmacology	Condition	Potency
Peroxidase C1A 3	Substrate 661

Conditions

Condition	Modality	Targets	Highest Phase	Product
Unknown 2,578

PubMed

Show entries

Title	Date	PubMed
Localization of intracellular lipid hydroperoxides using the tetramethylbenzidine reaction for transmission electron microscopy.	2002	12152190
Rapid detection of human rotavirus using colorimetric nucleic acid sequence-based amplification (NASBA)-enzyme-linked immunosorbent assay in sewage treatment effluent.	2002 Apr 23	12023091
Synthesis of chlorin e6-transferrin and demonstration of its light-dependent in vitro breast cancer cell killing ability.	2002 Mar	12038702
[Spectrophotometric determination of vanadium(V) through the formation of a tungstovanadophosphate-TMBPS charge transfer complex].	2002 Oct	12938434
An electrochemical ELISA procedure for the screening of 17beta-estradiol in urban waste waters.	2002 Oct	12430605

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Patents

Sample Use Guides

In Vivo Use Guide

Unknown

Route of Administration: Unknown

In Vitro Use Guide

The incubation mixture contained 10 uM of 3,5,3',5'-tetramethylbenzidine and 15 uM of hydrogen peroxide in acetate buffer, pH 5.0. At zero time, horseradish peroxidase was added (10 ng/ml).

Substance Class	Chemical
Record UNII	3B3T5CB8EO
Record Status	`Validated (UNII)`
Record Version

Show entries

Name

Type

Language

3,3',5,5'-TETRAMETHYLBENZIDINE

Systematic Name

English

COLORBURST BLUE

Brand Name

English

3,3',5,5'-TETRAMETHYLBENZIDINE [HSDB]

Common Name

English

3,3',5,5'-TETRAMETHYL(1,1'-BIPHENYL)-4,4'-DIAMINE

Systematic Name

English

POTASSIUM-BLUE MAX

Brand Name

English

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Code System

Code

Type

Description

WIKIPEDIA

3,3',5,5'-Tetramethylbenzidine

PRIMARY

HSDB

4331

PRIMARY

FDA UNII

3B3T5CB8EO

PRIMARY

MERCK INDEX

m11776

PRIMARY

PUBCHEM

41206

PRIMARY

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