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Leishmania brasiliensis five to eight day-old culture was used for testing. One ml contained 200,000 of organisms. The clear filtrate containing palitantin (0.1 ml) was suc- cessively applied on squares of chromato- graphic paper (1 cm ~) . The paper was then cut into pieces about 1 mm 2 and aseptically inserted into a tube containing 1 ml of Leish- mania brasiliensis culture. After 24 hours of cultivation at 28 C 0.2 ml was pipetted from each tube for the microscopic calculation of dead organisms per field. The pure compound affects Leishmania brasiliensis at concentrations of 50 to 100 ~g/ml.