| Stereochemistry | ACHIRAL |
| Molecular Formula | C21H20N3.Br |
| Molecular Weight | 394.308 |
| Optical Activity | NONE |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
[Br-].CC[N+]1=C(C2=CC=CC=C2)C3=C(C=CC(N)=C3)C4=C1C=C(N)C=C4
InChI
InChIKey=ZMMJGEGLRURXTF-UHFFFAOYSA-N
InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
| Molecular Formula | C21H19N3 |
| Molecular Weight | 313.3957 |
| Charge | 0 |
| Count |
MOL RATIO
1 MOL RATIO (average) |
| Stereochemistry | ACHIRAL |
| Additional Stereochemistry | No |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Optical Activity | NONE |
| Molecular Formula | BrH |
| Molecular Weight | 80.912 |
| Charge | 0 |
| Count |
MOL RATIO
1 MOL RATIO (average) |
| Stereochemistry | ACHIRAL |
| Additional Stereochemistry | No |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Optical Activity | NONE |
Ethidium is a DNA intercalating agent first discovered as and used as a veterenary trypanocide. A bromide salt is commonly used as a fluorescent tag in molecular biology. The fluorescene of ethidium bromide increased 21 fold upon binding to double-stranded RNA, 25 fold upon binding double stranded DNA. Because of the binding to DNA, ethidium bromide is a powerful inhibitor of DNA polymerase.
Originator
Approval Year
PubMed
Patents
Sample Use Guides
To investigate trypanocide activity, the drug in 1% or 2% aqueous solutions at a dose of 1 mg/kg was injected subcutaneously on the side of the thorax above the elbow, using cattle of the Shorthorn zebu type.
Route of Administration:
Other
The dye is usually incorporated into the gel and the electrophoresis buffer at 0.5 µg/ml. Note: Electrophoresis mobility of linear double-stranded DNA is reduced by approximately 15% in the presence of the dye. To stain after gel has been run, immerse gel in electrophoresis buffer or water containing EtBr (0.5 µg/ml) for 30-45 minutes at room temperature. Destaining is optional. Detection of very small amounts (<10 ng) of DNA is made easier if the background fluorescence caused by unbound EtBr is reduced by soaking the stained gel in water or 1 mM MgSO4 for 20 minutes at room temperature.
