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Details

Stereochemistry ACHIRAL
Molecular Formula C13H24N2O
Molecular Weight 224.3425
Optical Activity NONE
Defined Stereocenters 0 / 0
E/Z Centers 0
Charge 0

SHOW SMILES / InChI
Structure of 1,3-DICYCLOHEXYLUREA

SMILES

O=C(NC1CCCCC1)NC2CCCCC2

InChI

InChIKey=ADFXKUOMJKEIND-UHFFFAOYSA-N
InChI=1S/C13H24N2O/c16-13(14-11-7-3-1-4-8-11)15-12-9-5-2-6-10-12/h11-12H,1-10H2,(H2,14,15,16)

HIDE SMILES / InChI

Description

1,3-Dicyclohexylurea (DCU) is a potent endogenous inhibitor of soluble epoxide hydrolase (sEH), that has been found in human serum. Soluble epoxide hydrolase has been implicated in cardiovascular disease and inflammation in mammals. Endogenously produced 1,3-dicyclohexylurea may have physiological significance via regulation of soluble epoxide hydrolase activity in vivo.

Originator

Approval Year

Targets

Primary TargetPharmacologyConditionPotency
3.0 nM [IC50]

Conditions

ConditionModalityTargetsHighest PhaseProduct
Primary
Unknown

PubMed

Sample Use Guides

In Vivo Use Guide
From day 10 to day 14, rats were dosed orally with vehicle or 1,3-Dicyclohexylu at 30 mg/kg twice daily
Route of Administration: Oral
In Vitro Use Guide
Cyano(6-methoxy-naphthalen-2-yl)methyl trans-[(3-phenyloxyran-2-yl)methyl] carbonate (CMNPC) is the substrate used for the fluorescent assay. The assay was carried out using a 96 well-plate format. There was a lane for background with PB buffer (100 mM sodium phosphate, pH = 7.4, 200 µL) and two lanes for positive control. Each inhibitor IC50¬ was determined based on triplicate experiments. Each inhibitor (the highest desired concentration, 2 µL, DMSO) was added to the PB buffer (100 mM sodium phosphate, pH = 7.4, 150 µL) at the first set of wells. A series of 2-fold dilution was applied from the first set till to the last set of wells. hSEH solution (25 nM, 100 mM sodium phosphate, pH = 7.4, 20 µL) was added and mixed for 30s. The inhibitor-enzyme mixtures were mixed and incubated at 30 ˚C for 5 min. CMNPC solution (33 µM, 100 mM sodium phosphate, pH = 7.4, 30 µL) was added and mixed. The enzyme activity was measured based on the fluorescence increase.