Adults with acute diseases on day 1 - 1 g, then 200 mg / day. The duration of the course is 7-10 days. With chronic, long-term infections (with chronic lung diseases, rheumatism, kidney and urinary tract diseases) - 2 g in 1 dose once a week.

The bacteria Streptococcus faecium durans (Streptococcus faecalis ATCC 8043), Lactobacillus casei ATCC 7469, Pediococcus cereuisiae ATCC 8081, and Escherichia coli ATCC 10536 were used for activity evaluation. Amounts of 8 ml of the appropriate basal medium were placed in tubes and sterilized by autoclaving at 121 C for 10 min. Folic acid or calcium leucovorin diluted in 25% sterile glucose solution was added in 1-ml quantities. The final concentrations of folic acid were 0.002 ,ug/ml for S. faecium and 0.001 ,ug/ml for L. casei; the final concentration of calcium leucovorin was 0.001 ,ug/ml for P. cerevisiae. Stock solutions of PM in 0.04 N HCl and of CGT, TMP, and MTX in distilled water were sterilized by membrane filtration. SULFALENE dilutions in 1 ml of distilled water were added to give a final volume of 10 ml. Growth of the test organisms was read as turbidity in the Klett-Summerson photoelectric colorimeter. The results are expressed as the micromolar concentration required to produce 50% growth inhibition (ICs,) of the strain under the conditions used. Where sufficient drug was available, concentrations up to 1000 mkg/ml were tested; when this was not possible the highest concentration used was 100 mkg/ml.