| Stereochemistry | ACHIRAL |
| Molecular Formula | C12H14O3 |
| Molecular Weight | 206.2378 |
| Optical Activity | NONE |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 1 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
CCOC(=O)\C=C\C1=CC=C(OC)C=C1
InChI
InChIKey=DHNGCHLFKUPGPX-RMKNXTFCSA-N
InChI=1S/C12H14O3/c1-3-15-12(13)9-6-10-4-7-11(14-2)8-5-10/h4-9H,3H2,1-2H3/b9-6+
Ethyl Methoxycinnamate (EPMC , Ethyl-p-methoxycinnamate) is a major constituent of Kaempferia galanga. with nematicidal, mosquito repellent, anti-neoplastic and anti-microbial activity. In preclinical studies Ethyl Methoxycinnamate shows marked larvicidal, antifungal and anti-inflammatory activities as well as inhibitory activity on susceptible and multi-drug resistant (MDR) clinical M. tuberculosis strains isolated from tuberculosis patients as well. In an in vitro anti-inflammatory mechanistic study, Ethyl Methoxycinnamate was found to inhibit both COX-1 and COX-2.
Originator
Approval Year
PubMed
Sample Use Guides
Cytotoxicity of EPMC on human monocytic leukemic cell lines THP-1 was analysed by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium (MTT) assay. THP-1 cells were seeded in 96-well
microtitre plate at a density of 2×10^4 cells/well in quadruplicates. Various concentrations of EPMC (Ethyl Methoxycinnamate) in RPMI medium containing 10% foetal bovine serum (FBS) were added to the wells. Control wells did not contain EPMC. The cells were incubated at 37 °C in the presence of 5% CO2. After 24 h, 100 μl MTT (2.5 mg/ml stock) dissolved in RPMI was added and again incubated at 37 °C. After two hours, 100 μl of lysis buffer (20% SDS in 50% DMF) was added and incubated at 37 °C. The plate was read at 570 nm after 6 h and percentage viability of cells was calculated.
SUBSTANCE RECORD
