Rat: 100 mg/kg, 200 mg/kg, 400 mg/kg and 800 mg/kg

Cytotoxicity of EPMC on human monocytic leukemic cell lines THP-1 was analysed by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium (MTT) assay. THP-1 cells were seeded in 96-well microtitre plate at a density of 2×10^4 cells/well in quadruplicates. Various concentrations of EPMC (Ethyl Methoxycinnamate) in RPMI medium containing 10% foetal bovine serum (FBS) were added to the wells. Control wells did not contain EPMC. The cells were incubated at 37 °C in the presence of 5% CO2. After 24 h, 100 μl MTT (2.5 mg/ml stock) dissolved in RPMI was added and again incubated at 37 °C. After two hours, 100 μl of lysis buffer (20% SDS in 50% DMF) was added and incubated at 37 °C. The plate was read at 570 nm after 6 h and percentage viability of cells was calculated.