Unknown

Compound activity was evaluates in vitro inhibition of cell proliferation in various human tumor cells, including HeLa (cervical adenocarcinoma), HCT-116 (colon carcinoma), A375 (malignant melanoma), SK-OV-3 (ovarian adenocarcinoma), MDA-MB-231 (breast carcinoma), and PC-3 (prostate adenocarcinoma). Cells were trypsinized and counted and 3000-8000 cells were added to each well of a 96-well CytoStar tissue culture treated scintillating microplate. Cells were incubated for 24 hours in complete medium at 37 oC in an atmosphere containing 5% CO2. Next, 1  of compound 3n (CDK1/2 INHIBITOR III) in 100% DMSO was added to the wells of the plate. DMSO only was added to control wells. Cells were incubated for 24 more hours in complete medium at 37oC in an atmosphere containing 5% CO2.