Adult: As combination containing prilocaine hydrochloride 30 mg and felypressin 0.03 IU/ml: Usual dose: 1-5 ml, adjust according to response. Max: 10 ml. Child: As combination containing prilocaine hydrochloride 30 mg and felypressin 0.03 IU/ml: <10 yr: 1-2 ml; 10-18 yr: 1-5 ml, max 10ml. Adjust dose according to response.

Adenohypophyses, removed from decapitated rats, were divided into four segments of approximately equal size and incubated together for 150 min at 37 °C in 2-0 ml medium, ionically similar to cerebrospinal fluid containing ascorbic acid (1 mmol/1; pH 7-4), through which 95% O2 / 5% CO2 was passed continually. The medium was replaced after 120 and 135 min. The pituitary segments were then separated and incubated for a further 15 min, either in medium (1-0 ml) con¬ taining vasopressin-receptor agonist Felypressin and/or CRF-41 or in a corresponding volume of medium alone (controls). The medium was stored at -30 °C and its ACTH concentration determined subsequently