12β-Acetoxycimigenol 3-O-β-D-xylanopyranoside is a triterpenoid glycoside extracted from roots Cimicifuga racemosa, a plant used to treat a variety of ailments, including diarrhea, sore throat, rheumatism and menopausal disorders. The compound was also found in the roots of Actaea pachypoda and Cimicifuga fetida. The compound showed weak antibacterial activity in the agar diffusion assay and did not show any cytotoxicity against five human cancer cell lines.