Diethyl pyrocarbonate is modification reagent for histidine and tyrosine residues in proteins. Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total R

Ethyl 4-oxo-1-piperidinecarboxylate is used in organic synthesis. For example, in the synthesis of quinolones with antibacterial activity and DNA-gyrase inhibition properties, as well as in Loperamide synthesis. Ethyl 4-oxo-1-piperidinecarboxylate is a Loratadine impurity (0.01 to 0.8%). Stress studies applied on Loratadine were demonstrated that ethyl 4-oxo-1-piperidinecarboxylate (Impurity H) might represent a degradation byproduct.