4-Hydroxy-6-methyl-2-pyrone (also known as triacetic acid lactone) is a precursor of paragloboside contributing to insect and disease resistance of plant Gerbera hybrida and was recently demonstrated to be a potential platform chemical. 4-Hydroxy-6-methyl-2-pyrone is an isolated from the fungus, Hypoxylon investing, in 2014 which has not been extensively investigated for pharmacological activity. 4-Hydroxy-6-methyl-2-pyrone is a useful metabolite for analytical and bioassay dereplication of crude microbial extracts.