Tricine is an organic derivative of tris and glycine used in buffer solutions. It is a zwitterionic amino acid with a useful buffering range of pH 7.4-8.8 and is one of Good's buffering agents; Good first prepared tricine to buffer chloropast reactions. Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. Tricine–SDS-PAGE, based on Tricine-Tris buffer systems, is the commonly used SDS electrophoretic techniques for separating proteins. [99mTc]Tricine is a useful precursor complex for the radiolabeling of hydrazinonicotinate protein conjugates.