Unknown

Freshly isolated hepatocytes were prepared from two different rat livers and placed into primary culture. Forty-eight hours after plating, the cultures were incubated with Williams’ Medium E alone (UT), or containing 0.1% DMSO (vehicle control), 3 x 10_5 M of the active (Atorvastatin) or inactive (Ent-atorvastatin) enantiomer of atorvastatin (in DMSO). Twenty-four hours after treatment, hepatocytes were harvested for the preparation of total RNA, followed by analysis of CYP1A1, CYP2B, CYP3A, CYP4A, and HMG-CoA reductase (HMGRed) mRNA levels by Northern blot hybridization. After initial hybridization of membranes with cDNA probe, blots were rehybridized with a cDNA to 7S RNA, to permit normalization for loading and transfer among samples.