Male Dutch rabbits were treated with injections of 1.0, 2.0, and 4.0 mL of dibutyl oxalate and an equivalent volume of 0.5 M calcium chloride solution administered subcutaneously at a second injection site. Ophthalmoscopic observations were carried out on all animals prior to the first injection and on a daily basis thereafter. Animals were killed at 20 days or when signs of neurological impairment such as ataxia and stupor appeared. Animals treated with 1.0 mL of dibutyl oxalate showed few toxic signs and were killed at 20 days. Two of the five animals receiving 2.0 mL of dibutyl oxalate survived four and five days, respectively; the remaining three were killed at 20 days. The five animals receiving 4.0 mL died within three days, all exhibiting terminal signs of ataxia and stupor. A total of ten treated rabbits survived 3 days or more. Seven of these exhibited a "flecked retina"appearance o the fundus prior to death, and the clinical and pathologic findings in these animals were more extensive in those that survived up to 20 days. Histopathological examination revealed that the seven rabbits with flecked retinals demonstrated intracellular needle-like crystals within numerous cells of the RPE.

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