Unknown

Human embryonic kidney cells (HEK293), human hepatocellular carcinoma cells (HepG2), human neuroblastoma cells (SH-SY5Y and SK-N-SH), human leukemia T cells (Jurkat, clone E6-1), normal human foreskin fibroblasts (BJ) from a single donor (newborn male), normal human lung fibroblasts (MRC-5) from a single donor (male; 14 weeks gestation), normal human vascular endothelial cells (HUV-EC-C), rat hepatoma cells (H4-II-E), mouse neuroblastoma cells (N2a), and mouse fibroblast cells (NIH 3T3) were used for activity evaluation. After the cells were dispensed into 1536-well plates and incubated for 5–6 h, 23 nL of Diethanolamine oleate was transferred into the wells using a pin tool, resulting in final concentrations of 0.5 nM to 46 µM of Diethanolamine oleate and 0.45% DMSO. To achieve the highest final Diethanolamine oleate concentration of 92 µM (DMSO concentration 0.9%), 23 nL of Diethanolamine oleate was transferred twice from the highest concentration mother plate into each well of the assay plate; control plates using DMSO only at this highest concentration were also included. Caspase-3/7 activity was measured using a homogeneous luminescent method.