Over 5 visits Healthy volunteers and volunteers with impaired fasting glucose consumed 1 of 5 beverages with 50 g sucrose and varying levels of D-psicose (0,2.5, 5, 7.5 and 10 g). Beverages were presented in a random order and the subject and investigator were blind to the composition of the beverage. Subjects maintained 24-hour dietary records prior to each visit and abstained from consuming any calories for 8 hours prior to the visit. Venous blood samples were collected prior to consuming the beverage and at 30, 60, 90, and 10 min after.

Human ovarian adenocarcinoma cells (OVACAT-3) were maintained at 37 deg-C under an atmosphere of 5% CO2 in RPMI 1640 medium and supplemented with 10% fetal calf serum, 10 micro-g/mL insulin, 100 IU/mL penicillin, and 100 micro-g/mL streptomycin. Cell growth assay was initiated when the OVCAR-3 cells were in the logarithmic growth phase. Cells were seeded onto a 96-well plate with medium and cultured for 24 hours. Culture medium was refreshed including 50 mM of D-psicose. After 1 - 5 days, 10 micro L of MTT solution was added to each well and the plates incubated another 4 hours at 37 deg-C. The dose-dependent effect was further investigated by adding different concentrations of D-psicose (1, 5, 10, 20, 50 mM). The final MTT assay was performed after 1- 3 days. The inhibitory effect of D-allose on cancer cell proliferation was shown from day 1 and became more significant at day 5, in contrast to untreated cells. Furthermore, studies on the concentration effect of D-allose indicated that D-allose inhibits cells proliferation in a dose-dependent manner