Unknown

Vomifoliol was dissolved in 80% EtOH to a final concentration of 2 mg/mL. 5 μL of extract was impregnated into sterile Whatman No. 1 filter paper discs (6 mm diameter) and dried under a stream of sterile air. The bacterial pathogen N. gonorrhoeae (ATCC 49226 from ATCC as Kwik-STK™ plus microorganism) with viable counts reaching 10^8 CFU/mL was then streaked with sterile swabs onto Petri dishes containing chocolate (GC) agar base medium (CMO367, Oxoid Ltd., England) supplemented with 2% (w/v) hemoglobin and 1% (v/v) Vitox and the plates dried for 5 min at room temperature. The procedure was done in triplicate. Ciprofloxacin (1 μg/disc) was used as a positive control, while H2O and 80% EtOH were used as negative and solvent controls, respectively. Discs were applied to the dry surface of a Petri dish containing GC agar base medium and bacteria.