| Stereochemistry | ACHIRAL |
| Molecular Formula | C5H7N3 |
| Molecular Weight | 109.1292 |
| Optical Activity | NONE |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
C1CN2N=CC=C2N1
InChI
InChIKey=KPMVHELZNRNSMN-UHFFFAOYSA-N
InChI=1S/C5H7N3/c1-2-7-8-4-3-6-5(1)8/h1-2,6H,3-4H2
| Molecular Formula | C5H7N3 |
| Molecular Weight | 109.1292 |
| Charge | 0 |
| Count |
MOL RATIO
1 MOL RATIO (average) |
| Stereochemistry | ACHIRAL |
| Additional Stereochemistry | No |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Optical Activity | NONE |
IMPY is a potential single-photon emission computed tomography imaging agent for labeling of A-beta plaques of Alzheimer's disease. IMPY is a modified thioflavin derivative with good binding affinity to preformed synthetic Aβ1-40 aggregates, excellent brain uptake, and selective plaque labeling in AD transgenic mouse models and in postmortem AD brain sections. Unfortunately, the preliminary clinical data displayed a low signal-to-noise ratio, possibly due to excessive lipophilicity and low stability in vivo.
Approval Year
PubMed
Sample Use Guides
Brain frozen sections from the first group of three scrapie-infected mice and three age-matched controls were obtained. Coronal sections (20 μm thickness) were prepared using a cryostat and utilized for staining. The sections were incubated with 0.2 nM [125I]IMPY in PBS at room temperature for 1 h. The sections were then dipped in saturated Li2CO3 in 40% EtOH (two 3-min washes) and washed with 40% EtOH (two 2-min washes), followed by rinsing with water twice for 1 min. For in vitro competition of prion deposit labeling with [125I]IMPY, the brain sections of scrapie-infected mice were incubated with 0.2 nM [125I] IMPY in the presence of 0, 200 or 500 nM of nonradioactive IMPY (in 10% EtOH) at room temperature for 1 h. The labeled sections were then processed as described above. After drying, the labeled sections were exposed with BioMax MR film for 40 h together with an 125I microscale standard (GE Healthcare Biosciences). The films were then developed and analyzed using image analysis system Beta Vision (Biospace measures).