in mice: the day of the tail suspension test, experimental mice were transferred to the experiment room and allowed to acclimatize for 3–4 h. Mice were injected intraperitoneally with saline, EMDT (1, 2.5, 5, 10, or 15 mg/kg), fluoxetine (20 mg/kg), SB271046 (1, 5, or 10 mg/kg), or SB271046 (1, 5, or 10 mg/kg) combined with fluoxetine (20 mg/kg) 30 min before the tail suspension test trial.

EMDT increases the phosphorylation state of Thr34-DARPP-32 (Dopamine- and cAMP-regulated phosphoprotein) both in brain slices and in the intact brain. In vitro regulation of Thr34-DARPP-32 and Ser845-GluR1 phosphorylation by EDMTA (100 uM) was investigated in slices of neostriatum from wild-type and D1 knock-out mice.