Details
| Stereochemistry | ABSOLUTE |
| Molecular Formula | C16H28N4O4S |
| Molecular Weight | 372.483 |
| Optical Activity | UNSPECIFIED |
| Defined Stereocenters | 4 / 4 |
| E/Z Centers | 0 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
N[C@@H](CCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C(O)=O
InChI
InChIKey=BAQMYDQNMFBZNA-MNXVOIDGSA-N
InChI=1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1
| Molecular Formula | C16H28N4O4S |
| Molecular Weight | 372.483 |
| Charge | 0 |
| Count |
|
| Stereochemistry | ABSOLUTE |
| Additional Stereochemistry | No |
| Defined Stereocenters | 4 / 4 |
| E/Z Centers | 0 |
| Optical Activity | UNSPECIFIED |
Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Biocytin is also used in scientific research as a histological stain for nerve cells. Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Biocytin displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Biocytin can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope. In vitro injection of biocytin into explanted brain hemispheres provides a quick and easy method for tract tracing in developing brains. Biocytin can be used to measure the biotinidase activity and therefore diagnose biotinidase deficiency.
Originator
Approval Year
PubMed
| Title | Date | PubMed |
|---|---|---|
| Use of biocytin as neuroanatomic tracer in harvested human pancreas: a confocal laser scanning microscopy analysis. | 2002-05 |
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| NMDA receptor antagonists disinhibit rat posterior cingulate and retrosplenial cortices: a potential mechanism of neurotoxicity. | 2002-04-15 |
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| Axon arbors and synaptic connections of a vulnerable population of interneurons in the dentate gyrus in vivo. | 2002-04-15 |
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| Correlations between neuronal morphology and electrophysiological features in the rodent superficial dorsal horn. | 2002-04-01 |
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| Morphology and projection pattern of medial and dorsal pallial neurons in the frog Discoglossus pictus and the salamander Plethodon jordani. | 2002-04-01 |
|
| Anatomical, physiological, molecular and circuit properties of nest basket cells in the developing somatosensory cortex. | 2002-04 |
|
| Connections of the ventral telencephalon and tyrosine hydroxylase distribution in the zebrafish brain (Danio rerio) lead to identification of an ascending dopaminergic system in a teleost. | 2002-03-30 |
|
| Cell types and response properties of neurons in the ventral division of the medial geniculate body of the rabbit. | 2002-03-25 |
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| Colocalization of gamma-aminobutyric acid-like immunoreactivity and catecholamines in the feeding network of Aplysia californica. | 2002-03-25 |
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| Firing properties of axotomized central nervous system neurons recover after graft reinnervation. | 2002-03-18 |
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| A bisubstrate analog inhibitor of the carboxyltransferase component of acetyl-CoA carboxylase. | 2002-03-15 |
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| Biotin decreases retinal apoptosis and induces eye malformations in the early chick embryo. | 2002-03-04 |
|
| Detailed passive cable models of layer 2/3 pyramidal cells in rat visual cortex at different temperatures. | 2002-03-01 |
|
| Slow excitatory synaptic potentials evoked by distension in myenteric descending interneurones of guinea-pig ileum. | 2002-03-01 |
|
| Interconnections of auditory areas in the guinea pig neocortex. | 2002-03 |
|
| Visual cortical organization at the single axon level: a beginning. | 2002-03 |
|
| Sexual dimorphisms in a copulatory neuromuscular system in the green anole lizard. | 2002-02-11 |
|
| Development of individual axon arbors in a thalamocortical circuit necessary for song learning in zebra finches. | 2002-02-01 |
|
| Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish. | 2002-02-01 |
|
| Recovery from spinal cord injury: a new transection model in the C57Bl/6 mouse. | 2002-02-01 |
|
| Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells. | 2002-01-25 |
|
| Connections between the central posterior/prepacemaker nucleus and hypothalamic areas in the weakly electric fish Apteronotus leptorhynchus: evidence for an indirect, but not a direct, link. | 2002-01-21 |
|
| Dual cholinergic control of fast-spiking interneurons in the neostriatum. | 2002-01-15 |
|
| Neurons labeled from locomotor-related ventrolateral funiculus stimulus sites in the neonatal rat spinal cord. | 2002-01-14 |
|
| Firing patterns of inferior colliculus neurons-histology and mechanism to change firing patterns in rat brain slices. | 2002-01-04 |
|
| Serum biotinidase activity in children with chronic liver disease and its clinical significance. | 2002-01 |
|
| Somatostatin modulates Ca2+ currents in neostriatal neurons. | 2002 |
|
| In vitro formation of corticospinal synapses in an organotypic slice co-culture. | 2002 |
|
| Cholecystokinin-immunopositive basket and Schaffer collateral-associated interneurones target different domains of pyramidal cells in the CA1 area of the rat hippocampus. | 2002 |
|
| Early development of neuronal activity in the primate hippocampus in utero. | 2001-12-15 |
|
| Targeted PEG-based bioconjugates enhance the cellular uptake and transport of a HIV-1 TAT nonapeptide. | 2001-12-13 |
|
| Identification and characterization of an output neuron from the oscillatory molluscan olfactory network. | 2001-12-07 |
|
| Location of dye-coupled second order and of efferent vestibular neurons labeled from individual semicircular canal or otolith organs in the frog. | 2001-12-07 |
|
| Differential synaptic processing separates stationary from transient inputs to the auditory cortex. | 2001-12 |
|
| Synaptic targets of thalamic reticular nucleus terminals in the visual thalamus of the cat. | 2001-11-26 |
|
| P2X(7) receptors in the enteric nervous system of guinea-pig small intestine. | 2001-11-19 |
|
| Functionally independent columns of rat somatosensory barrel cortex revealed with voltage-sensitive dye imaging. | 2001-11-01 |
|
| NMDA receptor activation limits the number of synaptic connections during hippocampal development. | 2001-11 |
|
| Mutations in BTD causing biotinidase deficiency. | 2001-11 |
|
| Increased synaptic plasticity in the surround of visual cortex lesions in rats. | 2001-10-29 |
|
| AMPA receptor channels with long-lasting desensitization in bipolar interneurons contribute to synaptic depression in a novel feedback circuit in layer 2/3 of rat neocortex. | 2001-10-15 |
|
| A subpopulation of nervus terminalis neurons projects to the olfactory mucosa in Xenopus laevis. | 2001-10-01 |
|
| Electrophysiological analysis of suprachiasmatic nucleus projections to the ventrolateral preoptic area in the rat. | 2001-10 |
|
| Morphological changes in the neuronal substrate for the optokinetic reflex in albino ferrets. | 2001-10 |
|
| Recurrent CA1 collateral axons in developing rat hippocampus. | 2001-09-21 |
|
| Auditory and lateral line inputs to the midbrain of an aquatic anuran: neuroanatomic studies in Xenopus laevis. | 2001-09-17 |
|
| Compartmental models of rat cerebellar Purkinje cells based on simultaneous somatic and dendritic patch-clamp recordings. | 2001-09-01 |
|
| Physiology and morphology of intratelencephalically projecting corticostriatal-type neurons in pigeons as revealed by intracellular recording and cell filling. | 2001 |
|
| The fine organization of nigro-collicular channels with additional observations of their relationships with acetylcholinesterase in the rat. | 2001 |
|
| Regulation of the activity of hippocampal stratum oriens interneurons by alpha7 nicotinic acetylcholine receptors. | 2001 |
| Substance Class |
Chemical
Created
by
admin
on
Edited
Mon Mar 31 19:22:24 GMT 2025
by
admin
on
Mon Mar 31 19:22:24 GMT 2025
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| Record UNII |
G6D6147J22
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| Record Status |
Validated (UNII)
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| Record Version |
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| Code System | Code | Type | Description | ||
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576-19-2
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m2502
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G6D6147J22
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DTXSID30973172
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27870
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C013411
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83814
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BIOCYTIN
Created by
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