For Extra-amniotic Route A solution containing 250 μg/mL Prostin F2 alpha should be prepared. Insert a 12 to 14 French gauge Foley catheter with self-retaining 30 mL balloon through the cervix into the space between the fetal membranes and the uterine wall (extra-ovular or extra-amniotic), so that the balloon passes just beyond the internal os. Fill the balloon with 30 mL sterile water (a fine polyethylene catheter has also been used). The Prostin F2 alpha solution should then be instilled through the catheter. After filling the catheter system deadspace with a predetermined quantity of dilute solution, the initial dose should be 1 mL. Subsequent instillations should be 3 mL, unless side effects ensue, when the dose may be reduced to 1 or 2 mL or the interval between doses prolonged. Two hours should usually elapse between each installation and never less than 1 hour. For Intra-amniotic Route A transabdominal tap of the amniotic sac should be accomplished with an appropriate sized needle and at least 1 mL of amniotic fluid should be withdrawn, then 40 mg (8 mL) of Prostin F2 alpha is slowly injected into the amniotic sac. It is suggested that the first millilitre be injected very slowly to determine possible sensitivity prior to completing the total 40 mg dose. Do not inject medication in the case of a bloody tap. If within 24 hours of the initial dose the abortion process has not been established or completed (and in the presence of intact membranes), an additional 10-40 mg (2-8 mL) of Prostin F2 alpha may be administered.

Bobine luteal cells obtained at the mid-luteal stage (days 8-12 after ovulation) were cultured with prostaglandin F2α (PGF) (0.01, 0.1, 1 μM), interferon γ (IFNG) (0.05, 0.5, 5 nM) and tumor necrosis factor α (TNF) (0.05, 0.5, 0.5 nM) alone or in combination for 24 h. PGF and IFNG significantly increased the expression of MMP-1 mRNA. In addition, 1 μM PGF in combination with 5 nM IFNG stimulated MMP-1 and MMP-9 mRNA expression significantly more than either treatment alone. In contrast, IFNG significantly decreased the level of MMP-14 mRNA. The mRNA expression of TIMP-1, which preferentially inhibits MMP-1, was suppressed by 5 nM INFG. One μM PGF and 5 nM IFNG suppressed TIMP-2 mRNA expression.