Metastatic Testicular Tumors The usual Cisplatin Injection dose for the treatment of testicular cancer in combination with other approved chemotherapeutic agents is 20 mg/m2 IV daily for 5 days per cycle. Metastatic Ovarian Tumors The usual Cisplatin Injection dose for the treatment of metastatic ovarian tumors in combination with cyclophosphamide is 75 to 100 mg/m2 IV per cycle once every four weeks Advanced Bladder Cancer Cisplatin Injection should be administered as a single agent at a dose of 50 to 70 mg/m2 IV per cycle once every 3 to 4 weeks depending on the extent of prior exposure to radiation therapy and/or prior chemotherapy. All Patients Pretreatment hydration with 1 to 2 liters of fluid infused for 8 to 12 hours prior to a Cisplatin Injection dose is recommended. The drug is then diluted in 2 liters of 5% Dextrose in 1/2 or 1/3 normal saline containing 37.5 g of mannitol, and infused over a 6- to 8-hour period. If diluted solution is not to be used within 6 hours, protect solution from light. Do not dilute Cisplatin Injection in just 5% Dextrose Injection. Adequate hydration and urinary output must be maintained during the following 24 hours. A repeat course of Cisplatin Injection should not be given until the serum creatinine is below 1.5 mg/100 mL, and/or the BUN is below 25 mg/100 mL. A repeat course should not be given until circulating blood elements are at an acceptable level

Human ovarian carcinoma cells A2780 and cisplatin resistant SKOV3 were routinely cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, 100mg/ml penicillin and 50mg/ml streptomycin. The effect of SFN (sulforaphane), cisPt (cisplatin) or their combination on the survival of cells was determined by MTT assay. The cells were seeded at 1–2 10^3 cell density in 96-well culture plates. Each dose of tested compounds (added in the volume of 50ml) was tested in triplicate or quadruplicate. After 72 h, the cells were incubated with 50ml of MTT (1 mg/ml) and left in the dark at 37 C for an additional 4 h. Thereafter, the medium was removed, the formazan crystals were dissolved in 200ml of DMSO, and the absorbance was measured at 540 and 690 nm in xMarkTM Microplate Spectrophotometer (Bio-Rad Laboratories, Inc.).