Details
| Stereochemistry | ACHIRAL |
| Molecular Formula | C16H14N5.Cl |
| Molecular Weight | 311.769 |
| Optical Activity | NONE |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Charge | 0 |
SHOW SMILES / InChI
SMILES
[Cl-].CC1=CC=C(C=C1)N2N=C(N=[N+]2C3=CC=C(C)C=C3)C#N
InChI
InChIKey=SSJZAXOTLCJNLF-UHFFFAOYSA-M
InChI=1S/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15;/h3-10H,1-2H3;1H/q+1;/p-1
| Molecular Formula | ClH |
| Molecular Weight | 36.461 |
| Charge | 0 |
| Count |
|
| Stereochemistry | ACHIRAL |
| Additional Stereochemistry | No |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Optical Activity | NONE |
| Molecular Formula | C16H14N5 |
| Molecular Weight | 276.3159 |
| Charge | 1 |
| Count |
|
| Stereochemistry | ACHIRAL |
| Additional Stereochemistry | No |
| Defined Stereocenters | 0 / 0 |
| E/Z Centers | 0 |
| Optical Activity | NONE |
5-Cyano-2,3-di-p-tolyltetrazolium chloride (CTC) has been used to determine the in situ number of "active" bacteria in different ecosystems. It is a widely used and accepted dye in environmental and ecological studies. The redox dye CTC is a good vital stain for estimating aerobic bacteria with high metabolic activity due to their ability to reduce CTC to the fluorescent product. The reduction to the fluorescently active formazan form occurs by CTC scavenging for electrons from the components of the electron transport system or dehydrogenases. The dye has been used in conjunction with the nucleic acid staining dye DAPI (sc-3598) to quantitate viable bacteria. CTC has been used to measure the redox activity of tumor cells.
Approval Year
PubMed
| Title | Date | PubMed |
|---|---|---|
| Double staining (CTC-DAPI) for detection and enumeration of viable but non-culturable Campylobacter jejuni cells. | 1998-01-15 |
|
| Flow-cytometric determination of dehydrogenase activities in primary human gastrointestinal tumor cell lines. | 1994-02 |
|
| Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. | 1992-06 |
Sample Use Guides
In Vitro Use Guide
Curator's Comment: Direct microscopic enumeration of viable Campylobacter jejuni cells (ie, respiring bacteria) were performed in both culturable and non-culturable states. Five different C jejuni strains were used, including a reference strain, ATCC 33291. Cells from all five strains were incubated alone with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC).
Four hours of incubation with 5 mM CTC under a microaerobic atmosphere was found to be the optimal condition yielding the maximum number of respiring cells (both culturable and non-culturable).
| Substance Class |
Chemical
Created
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Edited
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| Record UNII |
BUS4P4I6FX
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| Record Status |
Validated (UNII)
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